Specimen

Blood/Bone Marrow: Collect 8 mL (5 mL min.) Blood/Bone marrow in Myco F / Lytic bottle available from LPL. Mix by gentle inversion 8–10 times. Ship at 18–22°C. CSF: Collect 2 mL (1 mL min.) CSF in a Red Top (No Additive) tube or sterile screw capped container. Do not use SST gel barrier tubes. Ship refrigerated. Pus/Body fluids (Pleural/Pericardial/Ascitic/ Synovial/Ocular) Aspirates /Semen/ BAL Bronchial washings: Submit as much as possible (1 mL min.) in sterile screw capped container. Ship refrigerated. Endometrial curettings/Tissue: Submit in sterile normal saline in a sterile screw capped container. Ship refrigerated. Swabs: Submit swabs in 1 mL sterile normal saline in sterile screw capped container. Ship refrigerated. Sputum/Urine: Submit 2 Spot (random) morning samples, 5-10 mL (1 mL min.) sputum/10 mL (5 mL min.) urine in sterile screw capped container. Do not pool the samples.Ship refrigerated. Gastric Lavage: Submit 5-10 mL (2 mL min.) gastric lavage in a sterile screw capped container. Ship refrigerated . Overnight fasting is mandatory.

Usage

This test includes speciation of Mycobacterium. Rapid automated culture allows the early recovery Mycobacteria within 7 days as compared to conventional culture which takes 4-6 weeks.